How to reconstitute 600mg and 1500mg glutathione

Glutathione vials hold far more powder than peptide vials, so they need far more water: 5 to 20 mL for a 600 mg vial and 10 to 30 mL for a 1500 mg vial, depending on the concentration you want. The technique is the same as for any lyophilized vial. The volumes, the chemistry and the storage are what change.

Why glutathione is different from a peptide vial

Most peptides we sell come in 5 or 10 mg vials. Our glutathione vials hold 600 mg and 1500 mg, which is 60 to 300 times as much powder. Glutathione is also small, 307.32 g/mol, so the molar amounts are large: a 600 mg vial is 1.95 millimoles and a 1500 mg vial is 4.88 millimoles. Peptide vials are usually counted in micromoles, a thousand times less.

Water volumes for a 600 mg vial

Water volumes for a 1500 mg vial

Before adding water, check how much the vial can physically hold. If the volume you want will not fit, dissolve at a higher concentration first, then transfer and dilute in a clean, larger sterile container. The glutathione calculator accepts any water volume if you need a figure that is not in the tables.

At these volumes, bacteriostatic water comes in larger sizes than most peptide work needs. Our bacteriostatic water is available in 3, 10, 20 and 30 mL vials, and the 30 mL size covers a 1500 mg vial at 50 mg/mL on its own. Bacteriostatic water vs sterile water covers which to use.

Adding the water

Glutathione dissolves readily, and the method is the one used for any lyophilized vial:

1. Let the vial reach room temperature before opening, so moisture does not condense on the powder.
2. Add the water slowly down the inside wall of the vial.
3. Swirl gently until the solution is clear. Do not shake.

How to reconstitute a lyophilized peptide walks through the same steps in more detail.

The solution is acidic, and that is normal

Reduced glutathione dissolved in water gives an acidic solution, roughly pH 3. The molecule carries two carboxylic acid groups, which is where the acidity comes from. An acidic reading is expected and is not a sign of a bad batch.

Oxidation: why reconstituted glutathione loses strength

Glutathione's active part is the thiol group on its cysteine. In solution, two glutathione molecules can join through that thiol to form glutathione disulfide (GSSG). It is the same chemistry that makes glutathione an antioxidant in cells: the thiol gives up electrons and the molecule pairs up as GSSG.

The reaction speeds up with:

• warmth
• air in the vial
• a less acidic solution
• trace metal ions

In practice, keep reconstituted glutathione in the fridge with the cap tight, and use it promptly. If it will not be used soon, split it into aliquots and freeze them rather than letting the whole vial sit. Dry, unopened vials keep best in the freezer. How to store research peptides has the temperatures.

Reading a glutathione lab report

HPLC separates reduced glutathione (GSH) from its oxidized form (GSSG), so a good glutathione report states purity for GSH specifically. It should show:

• purity at 99% or higher for reduced GSH
• an observed mass near 307.32
• a batch number that matches the vial, and a named lab

The QR code on every pepuro glutathione vial opens the report for its batch.

Every glutathione vial from pepuro ships free, from our Calgary, Toronto and Vancouver distribution centers, the same business day your e-Transfer clears, with a QR code on the vial that opens its independent, third-party lab report.

For research use only. Not for human or animal consumption.