Tool
Semaglutide reconstitution calculator
Semaglutide ships as 5 mg, 10 mg, 20 mg, 30 mg vials of lyophilized powder. Pick the size, type the water volume, read the concentration. The molecular weight is already filled in, so the molar result is there too.
- Concentration
- 2.5 mg/mL
- 2.5 µg/µL · 2500 µg/mL
- Molar
- 0.608 mM
- 607.7 µM
Concentration = peptide mass ÷ diluent volume. Molarity = (mg/mL ÷ g/mol) × 1000. Research use only.
Semaglutide at common volumes
| Vial | 1 mL | 2 mL | 3 mL | 5 mL |
|---|---|---|---|---|
| 5 mg | 5 mg/mL1.22 mM | 2.5 mg/mL0.608 mM | 1.67 mg/mL0.405 mM | 1 mg/mL0.243 mM |
| 10 mg | 10 mg/mL2.43 mM | 5 mg/mL1.22 mM | 3.33 mg/mL0.81 mM | 2 mg/mL0.486 mM |
| 20 mg | 20 mg/mL4.86 mM | 10 mg/mL2.43 mM | 6.67 mg/mL1.62 mM | 4 mg/mL0.972 mM |
| 30 mg | 30 mg/mL7.29 mM | 15 mg/mL3.65 mM | 10 mg/mL2.43 mM | 6 mg/mL1.46 mM |
Semaglutide vial sizes we sell, at four diluent volumes. Molarity uses 4113.58 g/mol. Other volumes
What the numbers mean for Semaglutide
Semaglutide has a molecular weight of 4113.58 g/mol (C187H291N45O59). So 5 mg is about 1.22 micromoles, whatever volume you dissolve it in. At 2.5 mg/mL that is 0.608 mM.
Each vial holds only powder, from 5 mg up to 30 mg, and no diluent. Add bacteriostatic water down the inside of the glass, swirl, and do not shake. The reconstitution guide covers the handling; this page covers the arithmetic.
Common questions
How much water do I add to a 5 mg vial of Semaglutide?
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Any volume that gives a concentration you can measure easily. 1 mL gives 5 mg/mL, 2 mL gives 2.5 mg/mL, 5 mL gives 1 mg/mL. The peptide amount stays 5 mg whatever you add.
What is the concentration of Semaglutide 5 mg in 2 mL?
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2.5 mg/mL, which is the same as 2.5 µg/µL. With a molecular weight of 4113.58 g/mol that is 0.608 mM.
Which Semaglutide vial sizes does the calculator cover?
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The ones we sell: 5 mg, 10 mg, 20 mg, 30 mg. Pick a chip or type any other mass.
Where does the Semaglutide molecular weight come from?
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The reference value on the product's Specifications tab, 4113.58 g/mol. The observed mass for the vial you receive is on its own lab report, which the QR code on the vial opens.
Related pages
- Buy Semaglutide5 mg, 10 mg, 20 mg, 30 mg. Free shipping, same-day dispatch, report on the vial.
- Is semaglutide available in Canada? What is sold hereResearch-grade semaglutide is sold in Canada for laboratory use. How it differs from Ozempic, how long it keeps outside the fridge, and how to check a vial.
- How long can retatrutide stay out of the fridge?Sealed, dry retatrutide is fine at room temperature in transit and briefly on the bench. Mixed retatrutide belongs in the fridge. Why, and which water to use.
- Cagrilintide vs Semaglutide: amylin analog vs GLP-1 analogPaired in research, not swapped. Cagrilintide acts at amylin and calcitonin receptors, semaglutide at GLP-1: different hormones, different masses.
- Semaglutide reconstitution: the numbers for the benchSemaglutide's numbers in one place: mass, the C18 chain that extends its half-life, vial sizes, concentration table and what its report should show.
- Semaglutide vs Retatrutide: one receptor or threeSemaglutide is a 31-residue GLP-1 analog. Retatrutide is a 39-residue triple agonist. 618 daltons and two receptors separate them.
- Semaglutide vs tirzepatide vs retatrutideThree incretin peptides, one, two and three receptors. How they differ by sequence, size and receptor target, and what tells them apart on a lab report.
- Semaglutide vs Tirzepatide: what is the difference?One receptor or two, 31 residues or 39, and a 700 dalton gap on the mass spec. How the two most-ordered GLP-1 research peptides differ, molecule by molecule.
- Calculator for any peptideType your own mass and molecular weight.
- Reconstitution and storage guidesThe handling around the numbers.
Research use only. Not for human or animal consumption.