Tool

Semaglutide reconstitution calculator

Semaglutide ships as 5 mg, 10 mg, 20 mg, 30 mg vials of lyophilized powder. Pick the size, type the water volume, read the concentration. The molecular weight is already filled in, so the molar result is there too.

Concentration
2.5 mg/mL
2.5 µg/µL · 2500 µg/mL
Molar
0.608 mM
607.7 µM

Concentration = peptide mass ÷ diluent volume. Molarity = (mg/mL ÷ g/mol) × 1000. Research use only.

Semaglutide at common volumes

Vial1 mL2 mL3 mL5 mL
5 mg5 mg/mL1.22 mM2.5 mg/mL0.608 mM1.67 mg/mL0.405 mM1 mg/mL0.243 mM
10 mg10 mg/mL2.43 mM5 mg/mL1.22 mM3.33 mg/mL0.81 mM2 mg/mL0.486 mM
20 mg20 mg/mL4.86 mM10 mg/mL2.43 mM6.67 mg/mL1.62 mM4 mg/mL0.972 mM
30 mg30 mg/mL7.29 mM15 mg/mL3.65 mM10 mg/mL2.43 mM6 mg/mL1.46 mM

Semaglutide vial sizes we sell, at four diluent volumes. Molarity uses 4113.58 g/mol. Other volumes

What the numbers mean for Semaglutide

Semaglutide has a molecular weight of 4113.58 g/mol (C187H291N45O59). So 5 mg is about 1.22 micromoles, whatever volume you dissolve it in. At 2.5 mg/mL that is 0.608 mM.

Each vial holds only powder, from 5 mg up to 30 mg, and no diluent. Add bacteriostatic water down the inside of the glass, swirl, and do not shake. The reconstitution guide covers the handling; this page covers the arithmetic.

Common questions

How much water do I add to a 5 mg vial of Semaglutide?+

Any volume that gives a concentration you can measure easily. 1 mL gives 5 mg/mL, 2 mL gives 2.5 mg/mL, 5 mL gives 1 mg/mL. The peptide amount stays 5 mg whatever you add.

What is the concentration of Semaglutide 5 mg in 2 mL?+

2.5 mg/mL, which is the same as 2.5 µg/µL. With a molecular weight of 4113.58 g/mol that is 0.608 mM.

Which Semaglutide vial sizes does the calculator cover?+

The ones we sell: 5 mg, 10 mg, 20 mg, 30 mg. Pick a chip or type any other mass.

Where does the Semaglutide molecular weight come from?+

The reference value on the product's Specifications tab, 4113.58 g/mol. The observed mass for the vial you receive is on its own lab report, which the QR code on the vial opens.

Related pages

Research use only. Not for human or animal consumption.